Product
소개
NuGel™ PBA Kit With Phenyl Boronic Acid NuGel™ Matrix
Glycoprotein Enrichment PBA Kit
• Cis-diol specific, enriches heterogeneous sets of glycoproteins
• Unmasks glycoproteins from high abundance proteins, most notably albumin
• Disposable, no column regeneration or cross-contamination
• Efficient new surface technology ideal for proteomic applications
• Enriches and purifies glycoproteins from blood, serum, plasma, tissue or cell
culture media.
• Removes > 90% of serum albumin (the non-glycosylated fraction)
• Sorbitol elution at pH 7.5 – 8.5
• Adaptable to a high-throughput 96-well format
Silica has been an industry standard as an advantageous matrix suitable for
high performance liquid chromatography. With NuGel™, non-specific sites
have been virtually eliminated making it an ideal support for affinity
purification. Through a proprietary polymer coating, silica is crosslinked
forming a reactive Poly-Epoxy functionality stable across a wide pH range
(pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity
products are derived.
The Phenyl Boronic Acid (PBA) ligand is immobilized through the NuGel™ poly-Epoxy linkage with attachment through the amino group. While various lectins bind to specific saccharide residues, the PBA ligand binds to the 1,2-cis-diol groups of biomolecules and enriches for heterogeneous sets of glycoproteins containing both N-linked and O-linked oligosaccharides. An easy and fast spin-filter format makes glycoprotein enrichment simple starting from 50µl serum, or 1-2 mg total protein.
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관련자료
Reference
Saliva
Gutiérrez, Ana, et al. "Influence of different sample preparation strategies on the proteomic identification of stress biomarkers in porcine saliva." BMC Veterinary Research 13.1 (2017): 375.
Gutiérrez, A. M., et al. "Detection and first characterization of an uncommon haptoglobin in porcine saliva of pigs with rectal prolapse by using boronic acid sample enrichment." animal 11.5 (2017): 845-853.