Product
소개
AlbuSorb™ PLUS Albumin + IgG Depletion Kit From Serum or Plasm
• > 400 µg total serum protein mass
(> 85% Albumin, >85% IgG depleted) from 25 µl serum prep
• Affinity-type equivalence, virtually no cross-reactivity with other
proteins
• Disposable, no column regeneration or cross-contamination
• Combines unique bead technology, not based on Blue-dye
affinity, with optimized Protein A
• Mild conditions maintains structural integrity and simple transfer
to secondary analysis
• The albumin and IgG depleted filtrate retains the enzymatic and
biological activity, along with enrichment of extracellular vesicle
(exosome) cargo
• Suitable for immunoassay, Western blot, 1 & 2D Electrophoresis,
enzyme assay, LC-MS
• Tested species include human, sheep, bovine, rabbit, mouse, rat
Poly-electrolytes are polymers with repeating units of stationary charges. AlbuSorb™ comes from a class of solid-phase, or surface-based, elastomeric poly-electrolytic surfaces that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. AlbuSorb™ combines with an optimized immobilized Protein A to create AlbuSorb™ PLUS. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination. AlbuSorb™ PLUS is supplied as a powder. Simply weigh, centrifuge and/or filter, and recover the albumin depleted serum in the supernatant.
☞ Click here to view AlbuSorb™ PLUS Product Sheet
사양
주문정보
관련자료
Cerebrospinal Fluid
Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270
Synovial fluid
Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex. Journal of Biological Chemistry.2012;287(11):8092-100
Serum
Zheng H, Soherwardy A, Roy S, Kuruc M, Avadhani S. AlbuVoid™ Enrichment & Antibody Depletion – Tackling the Challenges of Serum Proteomics Part II. Poster reprint first presented at 26th International Molecular Med TRI-CON, March 10-15, 2019, San Francisco, CA USA
Roy, Swapan, and Matthew Kuruc. "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors." Functional Proteomics. Humana Press, New York, NY, 2019. 41-54.
Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33
Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis
Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23
Urine
Zubiri, Irene, et al. "Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis." Journal of proteomics 96 (2014): 92-102.
Patent
Berggren, Per Olaf, Yang, Shao-Nian. 2012. Methods For Treating And/Or Limiting Development Of Diabetes.U.S. Patent 20120328630 Kind Code: A1, filed June 25, 2012, and issued December 27, 2012.