Product
소개
AlbuSorb™ Albumin Depletion Kit
• Removes >90% albumin from 30 mg albumin/ml sample
• Affinity-type equivalence, virtually no cross-reactivity with other proteins
• Bind and elute procedure - simply weigh powder, condition the sample,
centrifuge and/or filter, and recover the albumin depleted serum
• Economical surface technology, not based on blue-dye or immuno-affinity
chromatography
• Mild conditions maintain tertiary structure of proteins and simple transfer to
secondary analysis
• The albumin depleted filtrate retains the enzymatic and biological activity,
along with enrichment of extracellular vesicle (exosome) cargo
• Removes albumin from samples such as serum, plasma and from most
speciesincluding human, mouse, sheep, bovine, goat, rat, and calf
• The flow through fraction is compatible with LC-MS, activity based protein profiling
and proteomic studies.
AlbuSorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. With 25ul of starting sample, the yield of albumin depleted serum protein is 0.1-0.2 milligrams. AlbuSorb™ binds albumin from serum or plasma and is compatible with downstream proteomics methods such as protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS. Samples are also prepared for biomarker discovery, toxicological studies for new drugs, enzyme assays, protein profiling using SELDI analysis and cytokines research.
AlbuSorb™ binds to albumin, and serum proteins flow through. Removal of albumin allows enhanced detection of low abundance proteins. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.
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사양
주문정보
관련자료
References
Serum or plasma
Åvall, Karin. "In vivo and in vitro studies of apolipoprotein CIII in diabetes." (2017).
Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270