Product
소개
NAD+ Kinase Assay Kit
Drug target and enzyme assays
NAD+ kinase converts NAD+ into NADP+ by phosphorylation in the presence of ATP. NADP+ plays key roles in energy transduction and various biochemical process including DNA repair, protein modification and cell signaling. NAD+ kinase is an attractive antibacterial target.
The NAD+ kinase assay is based on measurement of ADP generated from the kinase reaction. The assay is fluorescence-based and can be carried out using regular black 96-well or 384-well plates or micro-cuvettes.
E. coli NAD+ Kinase Assay Kit Plus (Catalog No. NAK100KE)
Kit components: 600μl 10 x Buffer, 35μl 100x ATP, 35μl 100 x NAD+, 5μl 1000 x E.coli NADK, 35μl 100 x MUK reagent A, 35μl 100x MUK reagent B, 350μl 10 x fluorescence dye.
E. coli NAD+ Kinase Assay Kit Plus-500 (Catalog No. NAK500KE)
Kit components: 2 ml 10 x Buffer, 170μl 100x ATP, 170μl 100x NAD, 17μl 1000 x E.coli NADK, 170μl 100x MUK reagent A, 170μl 100x MUK reagent B, 1700μl 10 x fluorescence dye.