Product
소개
Bacterial Thymidylate Kinase Assay Kit
Drug target and enzyme assays
Thymidylate kinase is an important enzyme in the dTTP synthesis pathway for DNA synthesis. The function of this enzyme is to catalyze the phosphorylation of dTMP using ATP to form dTDP and ADP. Thymidylate kinase is an attractive antibacterial target.
The thymidylate kinase assay is based on measurement of ADP generated from the kinase reaction. The assay is fluorescence-based and can be carried out using regular black 96-well or 384-well plates or micro-cuvettes.
E. coli Thymidylate Kinase Assay Kit Plus (enzyme included) (Catalog No. TMK100KE)
Kit components: 600μl 10 x Buffer, 35μl 100x ATP, 35μl 100 x dTMP, 5μl 1000x E.coli TMK, 35μl 100x MUK reagent A, 35μl 100x MUK reagent B, 350μl 10 x fluorescence dye.
E. coli Thymidylate Kinase Assay Kit Plus-500 (enzyme included) (Catalog No. TMK500KE)
Kit components: 2 ml 10 x Buffer, 170μl 100 x ATP, 170μl 100x dTMP, 17μl 1000 x E.coli TMK, 170μl 100 x MUK reagent A, 170μl 100x MUK reagent B, 1700μl 10 x fluorescence dye.