Product
소개
Alcian Blue Stain Kit
Alcian Blue 2.5 pH is used to detect acid mucopolysaccharides in tissue samples, that cannot be detected with the habitual method of hematoxylin – eosin. Sulphured and carboxylated mucins can be visualized. Nuclear fast red is supplied as a counterstain, to visualize cell nucleus.
사용 및 시료 전처리 (Uses & Sample Preparation)
Samples do not require special preparation. This kit is design for research use only, it is not approved for human or animal use, or clinical diagnosis.
키트 구성 (Kit Components)
• 3% Glacial Acetic Acid
• Alcian Blue pH 2.5
• Nuclear Fast Red
염색 과정 (Procedure)
1. Deparaffinize sample and hydrate with distilled water
2. Add 3% Glacial acetic acid to the sample and remove excess
3. Add Alcian blue pH 2.5 (30 min)
4. Remove excess rinsing briefly with 3 % Glacial acetic acid (20 s). Rinse with tap water and deionized water two times (4 min)
5. Add Nuclear Fast Red (5 min)
6. Rinse in tap water and deionized water two times (4 min)
7. Dehydrate with graded ethanol, clear and coverslip
주문정보
KH07001-125 Alcian Blue Stain Kit 125 ml
KH07001-500 Alcian Blue Stain Kit 500 ml
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