Product
소개
ORAC Assay – Oxygen Radical Antioxidant Capacity
소개 (Description)
Antioxidant capacity is an overall ability of organisms or food to catch
free radicals and prevent their harmful effect. Antioxidative effect includes
protection of cells and cellular structures against harmful effect of free radicals,
especially oxygen and nitrogen. Antioxidants are substances with antioxidative
properties. Antioxidants are most commonly present in fruits and vegetables.
In addittion, other foods such as wine, meat or eggs contain these substances.
측정 원리 (Assay Principles)
The ORAC assay depends on the free radical damage to a fluorescent probe, such as fluorescein. As a result, there is a change of fluorescent intensity. Hence, the degree of change is indicative of the amount of radical damage. The presence of antioxidants results in an inhibition in the free radical damage to the fluorescent compound. This inhibition is observed as a preservation of the fluorescent signal.
It is possible to quantify the protection by calculating the area under the curve (AUC) from the experimental sample. After subtracting the AUC for the blank, the resultant difference would be the protection that the antioxidant compound confers. Trolox® (6-hydroxy-2,5,7,8-tetrametmethylchroman-2-carboxylic acid), a water-soluble vitamin E analog, is used as the calibration standard and ORAC results are expressed as Trolox® equivalents.
ORAC Data Analysis 보러 가기
주문정보
KF01004-100 ORAC Antioxidant Capacity Assay Kit 100 tests
KF01004-200 ORAC Antioxidant Capacity Assay Kit 200 tests
KF01004-400 ORAC Antioxidant Capacity Assay Kit 400 tests
관련자료